Comprehensive identification and characterization of abiotic stress and hormone responsive glycosyl hydrolase family 1 genes in Medicago truncatula

?-glucosidases (BGLUs) hydrolyze the ?-D-glycosidic bond with retention of anomeric configuration. BGLUs were associated many aspects plant physiological processes, in particular biotic and abiotic stresses through activation phytohormones defense compounds. However, studies on systematic analysis stress- or hormone-responsive BGLU genes are still rare. In this study, total 51 glycoside hydrolase family 1 identified genome model legume Medicago truncatula, they classified into five distinct clusters. Sequence alignments revealed several conserved characteristic motifs among these MtBGLU proteins. Analyses their putative signal peptides N-glycosylation site suggested that majority members have dual targeting to vacuole and/or chloroplast. Many regulatory elements possibly related genes. Moreover, Microarray qPCR analyses showed exhibited expression patterns various tissues, response different stress hormonal treatments. Notably, MtBGLU21, MtBGLU22, MtBGLU28, MtBGLU30 cluster I dramatically activated by NaCl, PEG, IAA, ABA, SA GA3 Collectively, our genome-wide characterization, evolutionary analysis, pattern play crucial roles cues M. truncatula.